Juventus owners collapsed from Coppa Italia on Wednesday with a 3-0 defeat in Atalanta, as striker Duvan Zapata scored twice to continue his electrical form.
Timothy Castan and Zapata hit at 37th and 39th minutes to stun a record 13-time winners, and Colombian striker Zapata scored one more time to organize a semifinal clash with Fiorentina.
Atalanta, who had a 2-2 draw in a draw against the leaders of Serie A Juventus in Bergamo in December, achieved another unexpected result, guaranteeing that Juve would not win the competition for the fifth consecutive season.
Castan caught Joao Kanchelu and shot home from the edge of the field to bring the hosts ahead, and Zapata pounced a second later.
Furious Juventus coach Massimiliano Allegri was sent off after throwing his jacket on the pitch after the second goal. However, the old woman was not forced to act, and she could not fix a single blow to the target in the first half.
Zapata scored second in the final stages, scoring 17 goals in the last 10 games in all competitions.